Leonhardt Lab - In Vivo Veritas

<body><table border="0" cellpadding="0" cellspacing="0" width="100%"><tr><td><p align="center"><font size="6"><strong>Leonhardt Lab</strong></font><br></p><p align="center"> </p></td></tr></table><table dir="ltr" border="0" cellpadding="0" cellspacing="0" width="100%"><tr><td valign="top"><p>Welcome to the colorful side of epigenetics!</p> <p>Obviously you are using a browser, which does not support frames! so better switch to one that does! i.e. <a target="_blank" href="http://www.spreadfirefox.com">Mozilla Firefox</a>...:-) </p> <p>This is how you can contact the people from the Leonhardt Lab:</p> <p>Prof. Dr. Heinrich Leonhardt 2180-74232<br> Dr. Lothar Schermelleh 2180-74230<br> Dr. Ulrich Rothbauer 2180-74287<br> Dr. Fabio Spada 2180-74230<br> Dr. Alessandro Brero 2180-74229<br> Dr. Akos Dobay 2180-74227<br> Anja Gahl 2180-74229<br> Kourosh Zolghadr 2180-74287<br> Oliver Mortusewicz 2180-74287<br> Karin Fellinger 2180-74227<br> Andrea Haemmer 2180-74227<br> Danny Meilinger 2180-7429<br> Katrin Schmidthals 2180-74287<br> <br>Fax 2180-74236</p> <p>This is what the people from the Leonhardt Lab published:</p> <p dir="ltr">2006<br> <br> Brero, A., Leonhardt, H., and Cardoso, M.C. (2006). Replication and translation of epigenetic information. Curr Top Microbiol Immunol<i> </i>301:21-44.<br> <BR> Perucca, P., Cazzalini, O., Mortusewicz, O., Necchi, D., Savio, M., Nardo, T., Stivala, L.A., Leonhardt, H., Cardoso, M.C., and Prosperi, E. (2006). Spatiotemporal dynamics of p21CDKN1A protein recruitment to DNA-damage sites and interaction with proliferating cell nuclear antigen. J Cell Sci.<br> <br> 2005<br> <br> Jambor de Sousa, U.L., Koss, M.D., Fillies, M., Gahl, A., Scheeder, M.R., Cardoso, M.C., Leonhardt, H., Geary, N., Langhans, W., and Leonhardt, M. (2005). CPT1alpha over-expression increases long-chain fatty acid oxidation and reduces cell viability with incremental palmitic acid concentration in 293T cells. Biochem Biophys Res Commun 338(2): 757-761.<br> <br> Hofmann, S., Rothbauer, U., Muhlenbein, N., Baiker, K., Hell, K., and Bauer, M.F. (2005). Functional and mutational characterization of human MIA40 acting during import into the mitochondrial intermembrane space. J Mol Biol 353(3): 517-28.<br> <br> Grunwald, D., Cardoso, M.C., Leonhardt, H., and Buschmann, V. (2005). Diffusion and Binding Properties Investigated by Fluorescence Correlation Spectroscopy (FCS). Curr Pharm Biotechnol 6(5): 381-6.<br> <br> Schermelleh, L., Spada, F., Easwaran, H.P., Zolghadr, K., Margot, J.B., Cardoso, M.C., and Leonhardt, H. (2005). Trapped in action: direct visualization of DNA methyltransferase activity in living cells. Nat Methods 2(10): 751-6.<br> <br> Grohmann, M., Spada, F., Schermelleh, L., Alenina, N., Bader, M., Cardoso, M.C., and Leonhardt, H. (2005). Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming. BMC Dev Biol 5(1): 18.<br> <br> Martin, R.M., Leonhardt, H., and Cardoso, M.C. (2005). DNA labeling in living cells. Cytometry A 67(1): 45-52.<br> <br> Sporbert, A., Domaing, P., Leonhardt, H., and Cardoso, M. C. (2005). PCNA acts as a stationary loading platform for transiently interacting Okazaki fragment maturation proteins. Nucleic Acids Res 33(11): 3521-8. <br> <br> Mortusewizc, O., L. Schermelleh, J. Walter, M.C. Cardoso and H. Leonhardt (2005). Recruitment of DNA methyltransferase I to DNA repair sites. PNAS 102(5): 8905-9.<br> <br> Spada, F., M. Chioda, E. M. Thompson (2005). Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles. J Cell Biochem 95(5): 885-901.<br> <br> Brero, A., H. P. Easwaran, D. Nowak, I. Grunewald, T. Cremer, H. Leonhardt and M.C. Cardoso (2005). Methyl CpG–binding proteins induce large-scale chromatin reorganization during terminal differentiation. J Cell Biol 169(5): 733-43.<br> <br> Spada, F., M. Vincent, E. M. Thompson (2005). Plasticity of histone modifications across the invertebrate to vertebrate transition: histone H3 lysine 4 trimethylation in heterochromatin. Chromosome Res 13(1): 57-72.<br> <br>Easwaran, H.P., H. Leonhardt and M.C. Cardoso (2005). Cell Cycle Markers for Live Cell Analyses. CellCycle 4(3): 453-5.<br> <br> Brister, P. C., Kuricheti, K. K., Buschmann, V., and Weston, K.D. (2005). Fluorescence correlation spectroscopy for flow rate imaging and monitoring- optimization, limitations and artifacts. Lab Chip 5(7): 785-791.<br> <br> Xiao, Y., Buschmann, V., and Weston, K.D. (2005). Scanning fluorescence correlation spectroscopy: a tool for probing microsecond dynamics of surface-bound fluorescent species. Anal Chem 77(1): 36-46.<br> <br>2004<br> <br>Solovei, I., L. Schermelleh, K. During, A. Engelhardt, S. Stein, C. Cremer and T. Cremer (2004). Differences in centromere positioning of cycling and postmitotic human cell types. Chromosoma 112(8): 410-23.<br><br>Sadoni, N., M. C. Cardoso, E. H. Stelzer, H. Leonhardt and D. Zink (2004). Stable chromosomal units determine the spatial and temporal organization of DNA replication. J Cell Sci 117(Pt 22): 5353-65.<br><br>Muhlenbein, N., S. Hofmann, U. Rothbauer and M. F. Bauer (2004). Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria. J Biol Chem 279(14): 13540-6.<br><br>Kuricheti, K.K., V. Buschmann and K.D. Weston (2004). Application of fluorescence correlation spectroscopy for velocity imaging in microfluidic devices. Appl Spectrosc 58:1180-6.<br><br>Gaudet, F., W. M. Rideout, 3rd, A. Meissner, J. Dausman, H. Leonhardt and R. Jaenisch (2004). Dnmt1 expression in pre- and postimplantation embryogenesis and the maintenance of IAP silencing. Mol Cell Biol 24(4): 1640-8.<br><br>Easwaran, H.P., L. Schermelleh, H. Leonhardt and M.C. Cardoso (2004). Replication-independent chromatin loading of Dnmt1 during G2 and M phases. EMBO Rep. 5:1181-6.<br><br>Dietzel, S., K. Zolghadr, C. Hepperger and A. S. Belmont (2004). Differential large-scale chromatin compaction and intranuclear positioning of transcribed versus non-transcribed transgene arrays containing beta-globin regulatory sequences. J Cell Sci 117(Pt 19): 4603-14.<br><br>Chioda, M., F. Spada, R. Eskeland and E.M. Thompson (2004). Histone mRNAs do not accumulate during S phase of either mitotic or endoreduplicative cycles in the chordate Oikopleura dioica. Mol Cell Biol. 24:5391-403.<br><br>2003<br><br>Walter, J., L. Schermelleh, M. Cremer, S. Tashiro and T. Cremer (2003). Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages. J Cell Biol 160(5): 685-97.<br><br>Margot, J. B., A. E. Ehrenhofer-Murray and H. Leonhardt (2003). Interactions within the mammalian DNA methyltransferase family. BMC Mol Biol 4(1): 7.<br><br>Gaudet, F., J. G. Hodgson, A. Eden, L. Jackson-Grusby, J. Dausman, J. W. Gray, H. Leonhardt and R. Jaenisch (2003). Induction of tumors in mice by genomic hypomethylation. Science 300(5618): 489-92.<br><br>Buschmann, V., K.D. Weston and M. Sauer (2003). Spectroscopic study and evaluation of red-absorbing fluorescent dyes. Bioconjug Chem 14:195-204.<br><br>Cupit, P.M., J.D. Hansen, A.S. McCarty, G. White, M. Chioda, F. Spada, S.T. Smale and C. Cunningham (2003). Ikaros family members from the agnathan Myxine glutinosa and the urochordate Oikopleura dioica: emergence of an essential transcription factor for adaptive immunity. J Immunol. 171:6006-13.<br><br>Cremer, T., M. Cremer, B. Wagler, J. Walter, and L. Schermelleh (2003). Imaging of human cell nuclei in space and time. Zellbiologie aktuell 29:21-23.<br> <br> Adhikary, A., Buschmann, V., Muller, C., and Sauer, M. (2003). Ensemble and single-molecule fluorescence spectroscopic study of the binding modes of the bis-benzimidazole derivative Hoechst 33258 with DNA. Nucleic Acids Res 31(8): 2178-2186. <br> <br>2002<br> <br>Sporbert, A., A. Gahl, R. Ankerhold, H. Leonhardt and M. C. Cardoso (2002). DNA polymerase clamp shows little turnover at established replication sites but sequential de novo assembly at adjacent origin clusters. Mol Cell 10(6): 1355-65.<br><br>Solovei, I., A. Cavallo, L. Schermelleh, F. Jaunin, C. Scasselati, D. Cmarko, C. Cremer, S. Fakan and T. Cremer (2002). Spatial preservation of nuclear chromatin architecture during three-dimensional fluorescence in situ hybridization (3D-FISH). Exp Cell Res 276(1): 10-23.<br><br>Quensel, C., J. Kramer, M. C. Cardoso and H. Leonhardt (2002). Smoothelin contains a novel actin cytoskeleton localization sequence with similarity to troponin T. J Cell Biochem 85(2): 403-9.<br><br>Hofmann, S., U. Rothbauer, N. Muhlenbein, W. Neupert, K. D. Gerbitz, M. Brunner and M. F. Bauer (2002). The C66W mutation in the deafness dystonia peptide 1 (DDP1) affects the formation of functional DDP1.TIM13 complexes in the mitochondrial intermembrane space. J Biol Chem 277(26): 23287-93.<br><br>Dietrich, A., V. Buschmann, C. Muller and M. Sauer (2002). Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data. J Biotechnol 82:211-31.<br><br>Derer, W., H. P. Easwaran, H. Leonhardt and M. C. Cardoso (2002). A novel approach to induce cell cycle reentry in terminally differentiated muscle cells. Faseb J 16(1): 132-3.<br><br>Cardoso, M. C. and H. Leonhardt (2002). Protein transduction: a novel tool for tissue regeneration. Biol Chem 383(10): 1593-9.<br><br>Schermelleh, L., and J. Walter (2002). Long term live cell confocal microscopy. G.I.T. Imaging & Microscopy 4:47-50 and (2003) BIOforum International 7: 48-50.<br><br>2001<br><br>Thompson, E.M., T. Kallesoe and F. Spada (2001). Diverse genes expressed in distinct regions of the trunk epithelium define a monolayer cellular template for construction of the oikopleurid house. Dev Biol. 238:260-73.<br><br><br>Spada, F., H. Steen, C. Troedsson, T. Kallesoe, E. Spriet, M. Mann and E.M. Thompson (2001). Molecular patterning of the oikoplastic epithelium of the larvacean tunicate Oikopleura dioica. J Biol Chem. 276:20624-32.<br><br>Schermelleh, L., I. Solovei, D. Zink and T. Cremer (2001). Two-color fluorescence labeling of early and mid-to-late replicating chromatin in living cells. Chromosome Res 9(1): 77-80.<br><br>Rothbauer, U., S. Hofmann, N. Muhlenbein, S. A. Paschen, K. D. Gerbitz, W. Neupert, M. Brunner and M. F. Bauer (2001). Role of the deafness dystonia peptide 1 (DDP1) in import of human Tim23 into the inner membrane of mitochondria. J Biol Chem 276(40): 37327-34.<br><br>Margot, J. B., M. C. Cardoso and H. Leonhardt (2001). Mammalian DNA methyltransferases show different subnuclear distributions. J Cell Biochem 83(3): 373-9.<br><br>Kramer, J., C. Quensel, J. Meding, M. C. Cardoso and H. Leonhardt (2001). Identification and characterization of novel smoothelin isoforms in vascular smooth muscle. J Vasc Res 38(2): 120-32.<br><br>Czerney, P., F. Lehmann, M. Wenzel, V. Buschmann, A. Dietrich and G.J. Mohr (2001). Tailor-made dyes for fluorescence correlation spectroscopy (FCS). Biol Chem 382:495-8.<br><br>2000<br><br>Paschen, S. A., U. Rothbauer, K. Kaldi, M. F. Bauer, W. Neupert and M. Brunner (2000). The role of the TIM8-13 complex in the import of Tim23 into mitochondria. Embo J 19(23): 6392-400.<br><br>Margot, J. B., A. M. Aguirre-Arteta, B. V. Di Giacco, S. Pradhan, R. J. Roberts, M. C. Cardoso and H. Leonhardt (2000). Structure and function of the mouse DNA methyltransferase gene: Dnmt1 shows a tripartite structure. J Mol Biol 297(2): 293-300.<br><br>Leonhardt, H. and M. C. Cardoso (2000). DNA methylation, nuclear structure, gene expression and cancer. J Cell Biochem Suppl Suppl 35: 78-83.<br><br>Leonhardt, H., H. P. Rahn, P. Weinzierl, A. Sporbert, T. Cremer, D. Zink and M. C. Cardoso (2000). Dynamics of DNA replication factories in living cells. J Cell Biol 149(2): 271-80.<br><br>Leonhardt, H., A. Sporbert and M. C. Cardoso (2000). Targeting regulatory factors to intranuclear replication sites. Crit Rev Eukaryot Gene Expr 10(2): 127-33.<br><br>Aguirre-Arteta, A. M., I. Grunewald, M. C. Cardoso and H. Leonhardt (2000). Expression of an alternative Dnmt1 isoform during muscle differentiation. Cell Growth Differ 11(10): 551-9.<br><br>1999 - 1991<br><br>Schermelleh, L., S. Thalhammer, W. Heckl, H. Posl, T. Cremer, K. Schutze and M. Cremer (1999). Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes. Biotechniques 27(2): 362-7.<br><br>Leonhardt, H., H. P. Rahn and M. C. Cardoso (1999). Functional links between nuclear structure, gene expression, DNA replication, and methylation. Crit Rev Eukaryot Gene Expr 9(3-4): 345-51.<br><br>Kramer, J., A. M. Aguirre-Arteta, C. Thiel, C. M. Gross, R. Dietz, M. C. Cardoso and H. Leonhardt (1999). A novel isoform of the smooth muscle cell differentiation marker smoothelin. J Mol Med 77(2): 294-8.<br><br>Engel, F. B., L. Hauck, M. C. Cardoso, H. Leonhardt, R. Dietz and R. von Harsdorf (1999). A mammalian myocardial cell-free system to study cell cycle reentry in terminally differentiated cardiomyocytes. Circ Res 85(3): 294-301.<br><br>Derer, W., H. P. Easwaran, C. W. Knopf, H. Leonhardt and M. C. Cardoso (1999). Direct protein transfer to terminally differentiated muscle cells. J Mol Med 77(8): 609-13.<br><br>Cardoso, M. C., A. Sporbert and H. Leonhardt (1999). Structure and function in the nucleus: subnuclear trafficking of DNA replication factors. J Cell Biochem Suppl 32-33: 15-23.<br><br>Cardoso, M. C. and H. Leonhardt (1999). DNA methyltransferase is actively retained in the cytoplasm during early development. J Cell Biol 147(1): 25-32.<br><br>Bauer, M. F., U. Rothbauer, N. Muhlenbein, R. J. Smith, K. Gerbitz, W. Neupert, M. Brunner and S. Hofmann (1999). The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom. FEBS Lett 464(1-2): 41-7.<br><br>Spada, F., A. Brunet, Y. Mercier, J.P. Renard, M.E. Bianchi and E.M. Thompson (1998). High mobility group 1 (HMG1) protein in mouse preimplantation embryos. Mech Dev. 76:57-66.<br><br>Reichert, A., U. Rothbauer and M. Morl (1998). Processing and editing of overlapping tRNAs in human mitochondria. J Biol Chem 273(48): 31977-84.<br><br>Leonhardt, H., H. P. Rahn and M. C. Cardoso (1998). Intranuclear targeting of DNA replication factors. J Cell Biochem Suppl 30-31: 243-9.<br><br>Gaudet, F., D. Talbot, H. Leonhardt and R. Jaenisch (1998). A short DNA methyltransferase isoform restores methylation in vivo. J Biol Chem 273(49): 32725-9.<br><br>Cardoso, M. C. and H. Leonhardt (1998). Protein targeting to subnuclear higher order structures: a new level of regulation and coordination of nuclear processes. J Cell Biochem 70(2): 222-30.<br><br>Falciola, L., F. Spada, S. Calogero, G. Langst, R. Voit, I. Grummt and M.E. Bianchi (1997). High mobility group 1 protein is not stably associated with the chromosomes of somatic cells. J Cell Biol. 137:19-26.<br><br>Cardoso, M. C., C. Joseph, H. P. Rahn, R. Reusch, B. Nadal-Ginard and H. Leonhardt (1997). Mapping and use of a sequence that targets DNA ligase I to sites of DNA replication in vivo. J Cell Biol 139(3): 579-87.<br><br>Barabino, S.M., F. Spada, F. Cotelli and E. Boncinelli (1997). Inactivation of the zebrafish homologue of Chx10 by antisense oligonucleotides causes eye malformations similar to the ocular retardation phenotype. Mech Dev. 63:133-43.<br><br>Tucker, K. L., D. Talbot, M. A. Lee, H. Leonhardt and R. Jaenisch (1996). Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene. Proc Natl Acad Sci U S A 93(23): 12920-5.<br><br>Rimini, R., A. Pontiggia, F. Spada, S. Ferrari, V.R. Harley, P.N. Goodfellow and M.E. Bianchi (1995). Interaction of normal and mutant SRY proteins with DNA. Philos Trans R Soc Lond B Biol Sci. 350:215-20.<br><br>Leonhardt, H. and M. C. Cardoso (1995). Targeting and association of proteins with functional domains in the nucleus: the insoluble solution. Int Rev Cytol 162B: 303-35.<br><br>Boncinelli, E., M. Gulisano, F. Spada and V. Broccoli (1995). Emx and Otx gene expression in the developing mouse brain. Ciba Found Symp. 193:100-16; discussion 117-26.<br><br>Leonhardt, H. and T. H. Bestor (1993). Structure, function and regulation of mammalian DNA methyltransferase. Exs 64: 109-19.<br><br>Cardoso, M. C., H. Leonhardt and B. Nadal-Ginard (1993). Reversal of terminal differentiation and control of DNA replication: cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication. Cell 74(6): 979-92.<br><br>Leonhardt, H., A. W. Page, H. U. Weier and T. H. Bestor (1992). A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei. Cell 71(5): 865-73.<br><br> Czank, A., R. Hauselmann, A. W. Page, H. Leonhardt, T. H. Bestor, W. Schaffner and M. Hergersberg (1991). Expression in mammalian cells of a cloned gene encoding murine DNA methyltransferase. Gene 109(2): 259-63.<br> </p></td></tr></table></body>